5x FIREPol® Master Mix Ready to Load (12.5 mM MgCl2)
Cat No - ABC-04-25-00125
5x FIREPol® Master Mix Ready to Load (12.5 mM MgCl2) is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). HOT FIREPol® Blend Master Mix contains two carefully optimized enzymes – HOT FIREPol® DNA polymerase and a proofreading polymerase. This enzyme blend has both the 5’→ 3’ exonuclease activity as well as the 3’→ 5’ proofreading activity. HOT FIREPol® Blend Master Mix exhibits an increased fidelity (up to five fold) compared to regular Taqpolymerase. In addition, the mix also allows amplification of longer fragments. Generated PCR products are compatible with blunt-end cloning procedures.