A-maize-ing Editing: Using CRISPR to Improve Crops

In this hands-on CRISPR-Cas9 simulation, students act as plant geneticists developing corn crops. They design guide RNAs, cleave DNA, and analyze results by gel electrophoresis. • Learn CRISPR-Cas9 applications with minimal equipment • Create and evaluate four guide RNAs using BLAST • Test gRNAs and control samples • Analyze DNA cleavage results using gel electrophoresis