Affinity Chromatography of Glucose Binding Proteins

Students prepare a Jack Bean seed extract, fractionate it by affinity chromatography, and elute glucose-binding protein. Biological activity is confirmed with an immunoblot enzyme assay. • Explore proteins and affinity chromatography • Conduct protein extraction and column separation • Isolate carbohydrate-binding lectin • Perform an immunoblot enzyme assay on eluted fractions • Complete three connected experiments