Antigen-Antibody Interaction: The Ouchterlony Procedure

Students explore antigen-antibody interactions using the Ouchterlony double diffusion method. Antibodies and antigens diffuse in agarose, forming visible precipitates that reveal their interactions. • Learn classic immunological techniques • Set up diffusion plates and observe precipitate formation • Understand equivalence zones • Identify reaction patterns: identity, partial identity, and non-identity