DNA Fingerprinting by Restriction Enzyme Patterns

Basic concepts of DNA fingerprinting are featured in this lab by comparing crime scene DNA with suspect DNAs. Fingerprint patterns are separated by agarose gel electrophoresis and the students determine who may have done-it! • Learn about DNA fingerprinting while solving a crime! • Introduce the biotechnologies of restriction enzyme digestion, PCR, and electrophoresis • Perform agarose gel electrophoresis to separate differently-sized DNA molecules • Load, run, and analyze