Fixing Genes, Fighting Disease: A Hands-On CRISPR Lab

Students simulate correcting a DMD-like mutation in bacteria using CRISPR to restore GFP fluorescence. The lab includes DNA ligation, bacterial transformation, protein extraction, and SDS-PAGE analysis, showing how mutations affect protein production and how gene therapy can reverse effects. • Explore CRISPR gene editing • Perform ligation, transformation, protein extraction, and SDS-PAGE • Connect molecular biology to human disease • Analyze fluorescence and protein gels