Investigation 9: Biotechnology - Restriction Enzyme Analysis of DNA
NEW & IMPROVED - Now for 10 Lab Groups! Analysis of Eco RI Cleavage Patterns of Lambda DNA This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments. • Introduce restriction enzymes and their ability to digest DNA at specific nucleotide sequences • Understand how electrophoresis separates differently-sized molecules • Perform agarose gel electrophoresis • Load, run, analyze, compare and size lambda DNA cut with HindIII, lambda DNA cut with EcoRI, and uncut lambda DNA
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NEW & IMPROVED - Now for 10 Lab Groups! Analysis of Eco RI Cleavage Patterns of Lambda DNA This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments. • Introduce restriction enzymes and their ability to digest DNA at specific nucleotide sequences • Understand how electrophoresis separates differently-sized molecules • Perform agarose gel electrophoresis • Load, run, analyze, compare and size lambda DNA cut with HindIII, lambda DNA cut with EcoRI, and uncut lambda DNA