Ion Exchange Chromatography
Most molecules carry a net charge between pH 2–10, and shifting the pH can dramatically change that charge. In this experiment, a two-chemical mixture is absorbed onto an ion-exchange column and separated during elution as pH alters each molecule’s behavior. • Learn ion-exchange chromatography • Explore how pH and charge affect molecular movement • Prepare a column and adjust pH to isolate different molecules • Special dyes allow easy visualization and optional spectrophotometer use
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Most molecules carry a net charge between pH 2–10, and shifting the pH can dramatically change that charge. In this experiment, a two-chemical mixture is absorbed onto an ion-exchange column and separated during elution as pH alters each molecule’s behavior. • Learn ion-exchange chromatography • Explore how pH and charge affect molecular movement • Prepare a column and adjust pH to isolate different molecules • Special dyes allow easy visualization and optional spectrophotometer use