Mapping of Restriction Sites on Plasmid DNA

DNA mapping is a common procedure used to determine the location of genes. In this experiment, DNA markers and pre-digested plasmid DNA fragments are mapped using agarose gel electrophoresis. • Understand and explore restriction enzymes using DNA mapping • Perform agarose gel electrophoresis to separate DNA fragments from three restriction enzyme digestions plus a standard marker • Determine the size of the DNA fragments generated • Construct a restriction enzyme map of the plasmid