Plant Pigment Chromatography and Photosynthesis

Chromatographic separation is performed by thin layer chromatography. Key reagents are included to demonstrate photosynthesis. • Understand and explore photosynthesis using biotechnology • Separate chloroplast pigments using thin-layer chromatography • Identify key pigments then measure and calculate their Rfs • Observe photosynthesis’ light dependent reactions using the color-changing dye DPIP • Perform a chromogenic assay to investigate factors that affect photosynthetic rate