Purification & Size Determination of Green & Blue Fluorescent Proteins

Students purify GFP and BFP from E. coli using their natural fluorescence. Fluorescent fractions are collected and optionally analyzed by SDS-PAGE to check purity and estimate protein size. • Explore protein purification and sizing • Learn GFP and BFP properties • Isolate proteins using column chromatography • Determine protein sizes with SDS-PAGE