Radial Immunodiffusion

Students use radial immunodiffusion to measure antigen levels. Antibody in agar reacts with antigen added to wells, forming a precipitate that radiates through the gel and can be quantified. • Explore antigen-antibody interactions and quantitative techniques • Perform a radial immunodiffusion experiment • Create a standard curve to determine unknown antigen concentration • Learn foundational immunology and immunodiffusion methods