Restriction Enzyme Cleavage of Plasmid and Lambda DNA

Plasmid and lambda DNA are pre digested with restriction enzymes endonucleases that recognize and cut double stranded DNA within or near defined base sequences. Digests are separated by agarose gel electrophoresis. • Introduce restriction enzymes and their ability to digest DNA at specific nucleotide sequences • Understand how electrophoresis separates differently sized molecules • Perform agarose gel electrophoresis • Load, run, analyze, and compare six restriction enzyme cleavage products.