Sickle Cell Gene Detection (DNA-based)

This experiment demonstrates how a single mutation in the hemoglobin gene causes Sickle Cell Anemia. Students use restriction enzyme analysis and gel electrophoresis to identify normal (HbA) and diseased (HbS) genes in a simulated patient test. • Learn how one DNA mutation affects health • Explore Mendelian genetics and molecular diagnostics • Perform and analyze agarose gel electrophoresis to detect hemoglobin gene mutations