Transformation of E. coli with Blue and Green Fluorescent Proteins

In this experiment, students explore bacterial transformation using E. coli and plasmid DNA, observing acquired traits like ampicillin resistance and fluorescence. • Learn genetic engineering and gene expression • Transform E. coli with pFluoroGreen™ or pFluoroBlue™ • Select transformed cells and calculate transformation efficiency • Use IPTG to demonstrate gene expression • Illustrate DNA → RNA → Protein → Trait concepts