Transformation of E. coli with Green Fluorescent Protein (GFP)

In this experiment, students explore bacterial transformation using E. coli and plasmid DNA, observing traits like ampicillin resistance and green fluorescence. • Learn genetic engineering and the central dogma • Transform E. coli with pFluoroGreen™ • Select transformed cells and calculate transformation efficiency • Use IPTG to demonstrate gene expression • High-efficiency protocol ensures clear, reliable results