What Is PCR and How Does It Work?

NGSS-aligned with MS-LS1. This simulation experiment demonstrates the process of DNA amplification by PCR and how the amplified product is detected by separating the reaction mixture by agarose gel electrophoresis. • Learn the biology behind polymerase chain reactions (PCR) and gel electrophoresis • Explore the relationship between PCR cycle number and amplified DNA quantities • Perform agarose gel electrophoresis to separate different sized dye molecules • Load, run, and analyze simulated DNA samples to observe size and concentration differences